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antibodies against shmt2  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc antibodies against shmt2
    Antibodies Against Shmt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/shmt2+33443s/SHMT2+Rabbit+mAb/pm41855880-270-6-9
    Average 94 stars, based on 35 article reviews
    antibodies against shmt2 - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Activation Assay:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Mass Spectrometry:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Produced:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Labeling:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Cytometry:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Control:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Knockdown:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Western Blot:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Expressing:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Gene Expression:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Transfection:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Liquid Chromatography with Mass Spectroscopy:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    shRNA:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Liposomes:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Staining:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Clinical Proteomics:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Flow Cytometry:

    Article Title: Mitochondrial folate metabolism-mediated α-linolenic acid exhaustion masks liver fibrosis resolution.
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco’s modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.

    Article Title: Mitochondrial folate metabolism–mediated α-linolenic acid exhaustion masks liver fibrosis resolution
    Article Snippet: Antibodies were purchased from the following resources: antibodies to phospho-SMAD2/3 (8828S), SMAD2/3 (8685S), SHMT1 (80715S), SHMT2 (33443S) from Cell Signaling Technology; antibodies to α-Actin (1A4) (ab150301) and TGFBR1(ab31013) from Abcam; antibodies to MTHFD1 (67670-1-1g), MTHFD1L (16113-1-AP) from Proteintech; antibodies to collagen 1α(I) from Southern Biotech; antibodies to GAPDH (AM1021B), Tubulin (M20005M) from Abmart; antibodies to α-Actin (1A4) (SC-32251) from Santa Cruz Biotechnology; antibody to MTHFD2 (GTX104990-S) from Gene Tex.. FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.FA-free RPMI 1640 (27016021), high glucose Dulbecco's modified Eagle medium (DMEM, 06-1055-57-1ACS) was purchased from Gibico.



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    ( A ) mRNA expression levels of the SHMT1 and <t>SHMT2</t> genes. ( B ) Expression level of the SHMT2 protein. ( C ) Prognostic analysis of high and low SHMT2 expression in HIF1A-positive and negative patients (TCGA data). ( D ) Evaluation of SHMT2 overexpression efficiency (OE) at the genetic level. ( E ) Assessment of SHMT2 overexpression efficiency (OE) at the protein level. ( F ) Expression levels of molecular markers directly associated with cell proliferation processes. ( G ) Expression levels of core members of the apoptosis pathway family. ns p > 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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    Image Search Results


    ( A ) mRNA expression levels of the SHMT1 and SHMT2 genes. ( B ) Expression level of the SHMT2 protein. ( C ) Prognostic analysis of high and low SHMT2 expression in HIF1A-positive and negative patients (TCGA data). ( D ) Evaluation of SHMT2 overexpression efficiency (OE) at the genetic level. ( E ) Assessment of SHMT2 overexpression efficiency (OE) at the protein level. ( F ) Expression levels of molecular markers directly associated with cell proliferation processes. ( G ) Expression levels of core members of the apoptosis pathway family. ns p > 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidation of the Role of SHMT2 in L-Serine Homeostasis in Hypoxic Hepa1-6 Cells

    doi: 10.3390/ijms252111786

    Figure Lengend Snippet: ( A ) mRNA expression levels of the SHMT1 and SHMT2 genes. ( B ) Expression level of the SHMT2 protein. ( C ) Prognostic analysis of high and low SHMT2 expression in HIF1A-positive and negative patients (TCGA data). ( D ) Evaluation of SHMT2 overexpression efficiency (OE) at the genetic level. ( E ) Assessment of SHMT2 overexpression efficiency (OE) at the protein level. ( F ) Expression levels of molecular markers directly associated with cell proliferation processes. ( G ) Expression levels of core members of the apoptosis pathway family. ns p > 0.05; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Equal amounts of total protein were separated by SDS-PAGE (Boster, Wuhan, China) and transferred to PVDF membranes (Merck Millipore, Burlington, MA, USA), which were blocked with skim milk powder (Yuan Ye, Hangzhou, China) for 1 h. The membranes were incubated with SHMT2 primary antibody (CST, New York, NY, USA) at 4 °C overnight.

    Techniques: Expressing, Over Expression

    ( A ) Analysis of apoptosis in Hepa1-6 cells under normoxic and hypoxic conditions using flow cytometry. ( B ) Detection of cell proliferation in Hepa1-6 cells under normoxic and hypoxic conditions using EdU staining. ( C ) High L-serine response of Hepa1-6 cells under hypoxia: Hypoxia leads to increased glycolysis, increased 3PHP and 3PS, and elevated L-serine production. A high L-serine pool causes the downregulation of amino acid synthesis genes such as PHGDH , PSAT1 , and PSPH , which inhibit the de novo synthesis of L-serine, as well as the downregulation of L-serine uptake genes such as SLC7A5 , which inhibits the exogenous uptake of L-serine. Additionally, downregulating SHMT2 inhibits the catabolism of L-serine into glycine and 5,10-MTHF . Together, these factors maintain high levels of L-serine stabilization in response to cellular metabolism under hypoxia. ns p > 0.05; ** p < 0.01, *** p < 0.001.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidation of the Role of SHMT2 in L-Serine Homeostasis in Hypoxic Hepa1-6 Cells

    doi: 10.3390/ijms252111786

    Figure Lengend Snippet: ( A ) Analysis of apoptosis in Hepa1-6 cells under normoxic and hypoxic conditions using flow cytometry. ( B ) Detection of cell proliferation in Hepa1-6 cells under normoxic and hypoxic conditions using EdU staining. ( C ) High L-serine response of Hepa1-6 cells under hypoxia: Hypoxia leads to increased glycolysis, increased 3PHP and 3PS, and elevated L-serine production. A high L-serine pool causes the downregulation of amino acid synthesis genes such as PHGDH , PSAT1 , and PSPH , which inhibit the de novo synthesis of L-serine, as well as the downregulation of L-serine uptake genes such as SLC7A5 , which inhibits the exogenous uptake of L-serine. Additionally, downregulating SHMT2 inhibits the catabolism of L-serine into glycine and 5,10-MTHF . Together, these factors maintain high levels of L-serine stabilization in response to cellular metabolism under hypoxia. ns p > 0.05; ** p < 0.01, *** p < 0.001.

    Article Snippet: Equal amounts of total protein were separated by SDS-PAGE (Boster, Wuhan, China) and transferred to PVDF membranes (Merck Millipore, Burlington, MA, USA), which were blocked with skim milk powder (Yuan Ye, Hangzhou, China) for 1 h. The membranes were incubated with SHMT2 primary antibody (CST, New York, NY, USA) at 4 °C overnight.

    Techniques: Flow Cytometry, Staining

    Primers used for qRT–PCR analysis.

    Journal: International Journal of Molecular Sciences

    Article Title: Elucidation of the Role of SHMT2 in L-Serine Homeostasis in Hypoxic Hepa1-6 Cells

    doi: 10.3390/ijms252111786

    Figure Lengend Snippet: Primers used for qRT–PCR analysis.

    Article Snippet: Equal amounts of total protein were separated by SDS-PAGE (Boster, Wuhan, China) and transferred to PVDF membranes (Merck Millipore, Burlington, MA, USA), which were blocked with skim milk powder (Yuan Ye, Hangzhou, China) for 1 h. The membranes were incubated with SHMT2 primary antibody (CST, New York, NY, USA) at 4 °C overnight.

    Techniques: Sequencing